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Image Search Results
Journal: Molecular and Cellular Biology
Article Title: A Novel Tel1/ATM N-Terminal Motif, TAN, Is Essential for Telomere Length Maintenance and a DNA Damage Response
doi: 10.1128/mcb.00326-08
Figure Lengend Snippet: FIG. 4. The TAN motif is required for the Tel1 DNA damage response. (A) Mutations in the Tel1 TAN motif reduce viability fol- lowing exposure to MMS. All strains were in the mec1 sml1 sae2 background. Cultures were grown at 30°C for 105 min with 0.01% MMS () or without MMS (), washed in YEPD, and then plated on YEPD and grown for 2 days at 30°C. (B) Mutations in the TAN motif reduce Rad53 phosphorylation following exposure to MMS. The West- ern blot of cell extracts from cells in panel A was probed for Rad53. Reduced mobility of Rad53 indicates phosphorylation (Rad53-P).
Article Snippet: Primary antibodies included mouse monoclonal anti-FLAG M2 (Sigma), mouse monoclonal anti-cmyc 9E10 (Covance),
Techniques: Phospho-proteomics